DH10beta F' DOT sbcC (donor strain)
(Bacterial strain #12082)

• Depositing Lab: Stephen Elledge
• Publication: Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7.

Backbone

• Vector backbone: na
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10beta F' DOT sbcC (donor strain)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: F' Tra operon

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Resource Information

• Supplemental Documents:
  • Elledge-pPRIME

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

F' contains a transfer operon (tra) and oriT is replaced with the tetracycline-resistant gene (tetR). This modified F' can mobilize the donor plasmid but not itself. The bacterial strain is DH10beta F' DOT sbcC (donor host strain): mcrA delta(mrr-hsdRMS-mcrBC) phi80lacZdeltaM15 delta-lacX74 deoR recA1 endA1 ara-delta139 delta(ara, leu)7697 galU galK lambda- rpsL nupG tonA umuC::pir116-frt F'(lac+ pro+ delta-oriT::Tc) sbcC::Frt.