Addgene - HA-Foxo1 (pCMV5) Plasmid Data

Plasmid 12142: HA-Foxo1 (pCMV5)

Gene/insert name:
Foxo1
Alt name:
Fkrh
Insert size:
2100
Species:
M. musculus (mouse)
GenBank ID:
NM_019739
Entrez Gene:
Foxo1 (AI876417, Afxh, FKHR, Fkhr1, Foxo1a)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Mutation:
Wild-type
Vector backbone:
pCMV5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4657
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Domenico Accili
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The forkhead transcription factor Foxo1 (Fkhr) confers insulin sensitivity onto glucose-6-phosphatase expression. Nakae et al (J Clin Invest. 2001 Nov . 108(9):1359-67. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12142" in your Materials and Methods section.