Addgene - GFP-Foxo1 Plasmid Data

Plasmid 17551: GFP-Foxo1

Gene/insert name:
Foxo1
Alt name:
pEGFP-N1 Foxo1
Insert size:
1960
Species:
M. musculus (mouse)
Entrez Gene:
Foxo1 (AI876417, Afxh, FKHR, Fkhr1, Foxo1a)
Fusion protein or tag:
EGFP
Terminal:
C terminal on backbone
Vector backbone:
pEGFP-N1
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
KpnI
Site destroyed during cloning:
Yes
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (3)
Principal Investigator:
Domenico Accili
Terms and Licenses:
MTA

Comments:

Has polymorphism L619P

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Nuclear trapping of the forkhead transcription factor FoxO1 via Sirt-dependent deacetylation promotes expression of glucogenetic genes. Frescas et al (J Biol Chem. 2005 May 27. 280(21):20589-95. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17551" in your Materials and Methods section.