pCMV6M-Pak1 P13A
(Plasmid #12207)

• Depositing Lab: Jonathan Chernoff
• Publication: Galisteo et al J Biol Chem. 1996 Aug 30. 271(35):20997-1000.

Backbone

• Vector backbone: pCMV6
• Backbone size w/o insert (bp): 4900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pak1
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: P13A: disrupts first polyproline region of Pak1
• Entrez Gene: PAK1 (a.k.a. IDDMSSD, PAKalpha, alpha-PAK, p65-PAK)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TCGTTTAGTGAACCGTCAG
• 3′ sequencing primer: GGAACTTCCAAGGCCAGGA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Pak1 may contain a G401S mutation. Depositor states that this mutation should not affect plasmid function.