Addgene - pLOX-CW-CRE Plasmid Data

Plasmid 12238: pLOX-CW-CRE

Gene/insert name:
CRE
Mutation:
CMV promoter is upstream of nls-CRE. LoxP in 3' LTR.
Vector backbone:
pHR
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, Cre/Lox
Backbone size w/o insert (bp):
9886
5' sequencing primer:
CMV forward List of Sequencing Primers
3' sequencing primer:
SP6 for lox site
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (3)
Principal Investigator:
Didier Trono
Terms and Licenses:
MTA

Comments:

Please note that the full sequence for this plasmid is approximated and not fully verified. ALTHOUGH THE SEQUENCE OF THIS PLASMID SUGGESTS IT'S FULL SIZE IS 9.9KB, OUR TEST DIGEST SHOWS IT IS ACTUALLY 11.5KB. NONETHELESS, OUR CUSTOMERS HAVE BEEN ABLE TO MAKE FUNCTIONAL VIRAL PARTICLES USING THIS PLASMID.

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lentivector-mediated transfer of Bmi-1 and telomerase in muscle satellite cells yields a duchenne myoblast cell line with long-term genotypic and phenotypic stability. Cudre-Mauroux et al (Hum Gene Ther. 2003 Nov 1. 14(16):1525-33. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12238" in your Materials and Methods section.