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pLOX-CW-CRE
(Plasmid #12238)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12238 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHR
  • Backbone size w/o insert (bp) 9886
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRE
  • Mutation
    CMV promoter is upstream of nls-CRE. LoxP in 3' LTR.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer SP6 for lox site
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that the full sequence for this plasmid is approximated and not fully verified. ALTHOUGH THE SEQUENCE OF THIS PLASMID SUGGESTS IT'S FULL SIZE IS 9.9KB, OUR TEST DIGEST SHOWS IT IS ACTUALLY 11.5KB. NONETHELESS, OUR CUSTOMERS HAVE BEEN ABLE TO MAKE FUNCTIONAL VIRAL PARTICLES USING THIS PLASMID.

Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLOX-CW-CRE was a gift from Didier Trono (Addgene plasmid # 12238 ; http://n2t.net/addgene:12238 ; RRID:Addgene_12238)
  • For your References section:

    Lentivector-mediated transfer of Bmi-1 and telomerase in muscle satellite cells yields a duchenne myoblast cell line with long-term genotypic and phenotypic stability. Cudre-Mauroux C, Occhiodoro T, Konig S, Salmon P, Bernheim L, Trono D. Hum Gene Ther. 2003 Nov 1. 14(16):1525-33. 10.1089/104303403322495034 PubMed 14577914