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Plasmid 12245: pLOX-TERT-iresTK
  • 2nd generation lentiviral vector for reversible immortalization of human primary cells

  • TERT, HSV1-TK

  • TERT

  • HSV-1 thymidine kinase

  • H. sapiens (human)

  • TERT (CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)

  • LoxP in 3' LTR.

  • pHR'
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, Cre/Lox

  • 13115

  • CMV Fwd for hTert List of Sequencing Primers

  • SP6 primer rev for LoxP

  • Ampicillin

  • Stbl3

  • 37

  • Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C

  • High Copy

  • View sequences (5)
  • View map

  • Didier Trono

  • UBMTA

Comments: 

CMV promoter is upstream of the bicistronic coding cassette with hTert and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.

Please note that the full sequence for this plasmid is approximated and not fully verified (for instance, Addgene's SP6 sequence shows a large insert not reported in the full sequence, which includes an additional EcoRI site). Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Reversible immortalization of human primary cells by lentivector-mediated transfer of specific genes. Salmon et al (Mol Ther. 2000 Oct . 2(4):404-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12245" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only