pLOX-Ttag-iresTK
(Plasmid #12246)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pHR'
  • Backbone size w/o insert (bp) 12290
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    SV40 Large T, HSV1-TK
  • Alt name
    SV40 Large T
  • Alt name
    HSV-1 thymidine kinase
  • Species
    Simian virus
  • Mutation
    LoxP in 3' LTR.
  • Entrez Gene
    SV40gp6 (a.k.a. SV40gp6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CMV Fwd for LargeT
  • 3′ sequencing primer SP6 primer rev for LoxP
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

CMV promoter is upstream of the bicistronic coding cassette with SV40-Large T and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.

Please note that the full sequence for this plasmid is approximated and not fully verified--there will be discrepancies between Addgene's quality control sequence and the theoretical sequence from the depositor. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLOX-Ttag-iresTK was a gift from Didier Trono (Addgene plasmid # 12246)
  • For your References section:

    Reversible immortalization of human primary cells by lentivector-mediated transfer of specific genes. Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D. Mol Ther. 2000 Oct . 2(4):404-14. 10.1006/mthe.2000.0141 PubMed 11020357