Addgene - pLOX-Ttag-iresTK Plasmid Data

Plasmid 12246: pLOX-Ttag-iresTK

Gene/insert name:
SV40 Large T, HSV1-TK
Alt name:
SV40 Large T
Alt name:
HSV-1 thymidine kinase
Species:
Simian virus
Entrez Gene:
SV40gp6 (SV40gp6)
Mutation:
LoxP in 3' LTR.
Vector backbone:
pHR'
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, Cre/Lox
Backbone size w/o insert (bp):
12290
5' sequencing primer:
CMV Fwd for LargeT List of Sequencing Primers
3' sequencing primer:
SP6 primer rev for LoxP
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
High or low copy:
High Copy
Sequence: View sequences (5)
Map:
View map
Principal Investigator:
Didier Trono
Terms and Licenses:
MTA

Comments:

CMV promoter is upstream of the bicistronic coding cassette with SV40-Large T and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.

Please note that the full sequence for this plasmid is approximated and not fully verified--there will be discrepancies between Addgene's quality control sequence and the theoretical sequence from the depositor. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Reversible immortalization of human primary cells by lentivector-mediated transfer of specific genes. Salmon et al (Mol Ther. 2000 Oct . 2(4):404-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12246" in your Materials and Methods section.