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Plasmid 12252: pRRLSIN.cPPT.PGK-GFP.WPRE
  • None

  • pRRL
    (Search Vector Database)

  • Naldini lab

  • Mammalian Expression, Lentiviral

  • See map List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C

  • High Copy

  • pRRL came from Naldini lab (JVI 98). R. Zufferey from Trono lab inserted the SIN in 98 and then WPRE in 99. Zennou et al finally inserted the cPPT.

  • View sequences (4)
  • Didier Trono

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12252" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only