1
Plasmid 12252: pRRLSIN.cPPT.PGK-GFP.WPRE
  • None

  • pRRL
    (Search Vector Database)

  • Naldini lab

  • Mammalian Expression, Lentiviral

  • See map List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C

  • High Copy

  • pRRL came from Naldini lab (JVI 98). R. Zufferey from Trono lab inserted the SIN in 98 and then WPRE in 99. Zennou et al finally inserted the cPPT.

  • View sequences (4)
  • Didier Trono

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12252" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only