|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12252||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerNaldini lab
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer See map (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypRRL came from Naldini lab (JVI 98). R. Zufferey from Trono lab inserted the SIN in 98 and then WPRE in 99. Zennou et al finally inserted the cPPT.
Terms and Licenses
Articles Citing this Plasmid
Please visit the Trono lab website http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
Addgene NGS results identified an IS4-like element in the LTR-packaged region, upstream of the insert, between the RRE and cPPT elements. Because the element is not located in functional sequences of the plasmid, we do not expect it to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRRLSIN.cPPT.PGK-GFP.WPRE was a gift from Didier Trono (Addgene plasmid # 12252)