Addgene - pRRLSIN.cPPT.PGK-GFP.WPRE Plasmid Data

Plasmid 12252: pRRLSIN.cPPT.PGK-GFP.WPRE

Gene/insert name:
None
Vector backbone:
pRRL
(Search Vector Database)
Backbone manufacturer:
Naldini lab
Vector type:
Mammalian Expression, Lentiviral
5' sequencing primer:
See map List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pRRL came from Naldini lab (JVI 98). R. Zufferey from Trono lab inserted the SIN in 98 and then WPRE in 99. Zennou et al finally inserted the cPPT.
Sequence: View sequences (4)
Principal Investigator:
Didier Trono
Terms and Licenses:
MTA

Comments:

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12252" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with