Addgene - psPAX2 Plasmid Data

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Related Plasmids

Didier Trono Lab Plasmids

This is commonly
requested with

pMD2.G

pLVTHM

pLKO.1 - TRC cloning vector

pLVCT-tTR-KRAB

scramble shRNA

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Plasmid 12260: psPAX2

Gene/insert name:		None
Insert size (bp):		Unknown
Vector backbone:		psPAX2 (Search Vector Database)
Type of vector:		Mammalian expression,Lentiviral,Packaging
Backbone size (bp):		10703
5' Sequencing primer:		See map (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Didier Trono
Terms and Licenses:		MTA

Comments: 2nd generation packaging vector. Known to work with many of the Aebischer and Trono lab lentiviral vectors, as well as the pLKO system. Produces higher titer than pCMV-dR8.2 dvpr.

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab (http://tronolab.epfl.ch) for cloning strategies, protocols, publications, and more. See LentiWeb (http://www.lentiweb.com) for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

84 - 371

Orf frame 1

1533 - 802

pCAG_F_primer

1678 - 1697

Orf frame 1

1780 - 3282

Gag_HIV(variant)

1780 - 3282

Orf frame 3

3348 - 6086

Orf frame 3

atgaatttgccaggaagatggaaaccaaaaatgatagggggaattggaggttttatcaaa
gtaggacagtatgatcagatactcatagaaatctgcggacataaagctataggtacagta
ttagtaggacctacacctgtcaacataattggaagaaatctgttgactcagattggctgc
actttaaattttcccattagtcctattgagactgtaccagtaaaattaaagccaggaatg
gatggcccaaaagttaaacaatggccattgacagaagaaaaaataaaagcattagtagaa
atttgtacagaaatggaaaaggaaggaaaaatttcaaaaattgggcctgaaaatccatac
aatactccagtatttgccataaagaaaaaagacagtactaaatggagaaaattagtagat
ttcagagaacttaataagagaactcaagatttctgggaagttcaattaggaataccacat
cctgcagggttaaaacagaaaaaatcagtaacagtactggatgtgggcgatgcatatttt
tcagttcccttagataaagacttcaggaagtatactgcatttaccatacctagtataaac
aatgagacaccagggattagatatcagtacaatgtgcttccacagggatggaaaggatca
ccagcaatattccagtgtagcatgacaaaaatcttagagccttttagaaaacaaaatcca
gacatagtcatctatcaatacatggatgatttgtatgtaggatctgacttagaaataggg
cagcatagaacaaaaatagaggaactgagacaacatctgttgaggtggggatttaccaca
ccagacaaaaaacatcagaaagaacctccattcctttggatgggttatgaactccatcct
gataaatggacagtacagcctatagtgctgccagaaaaggacagctggactgtcaatgac
atacagaaattagtgggaaaattgaattgggcaagtcagatttatgcagggattaaagta
aggcaattatgtaaacttcttaggggaaccaaagcactaacagaagtagtaccactaaca
gaagaagcagagctagaactggcagaaaacagggagattctaaaagaaccggtacatgga
gtgtattatgacccatcaaaagacttaatagcagaaatacagaagcaggggcaaggccaa
tggacatatcaaatttatcaagagccatttaaaaatctgaaaacaggaaaatatgcaaga
atgaagggtgcccacactaatgatgtgaaacaattaacagaggcagtacaaaaaatagcc
acagaaagcatagtaatatggggaaagactcctaaatttaaattacccatacaaaaggaa
acatgggaagcatggtggacagagtattggcaagccacctggattcctgagtgggagttt
gtcaatacccctcccttagtgaagttatggtaccagttagagaaagaacccataatagga
gcagaaactttctatgtagatggggcagccaatagggaaactaaattaggaaaagcagga
tatgtaactgacagaggaagacaaaaagttgtccccctaacggacacaacaaatcagaag
actgagttacaagcaattcatctagctttgcaggattcgggattagaagtaaacatagtg
acagactcacaatatgcattgggaatcattcaagcacaaccagataagagtgaatcagag
ttagtcagtcaaataatagagcagttaataaaaaaggaaaaagtctacctggcatgggta
ccagcacacaaaggaattggaggaaatgaacaagtagatgggttggtcagtgctggaatc
aggaaagtactatttttagatggaatagataaggcccaagaagaacatgagaaatatcac
agtaattggagagcaatggctagtgattttaacctaccacctgtagtagcaaaagaaata
gtagccagctgtgataaatgtcagctaaaaggggaagccatgcatggacaagtagactgt
agcccaggaatatggcagctagattgtacacatttagaaggaaaagttatcttggtagca
gttcatgtagccagtggatatatagaagcagaagtaattccagcagagacagggcaagaa
acagcatacttcctcttaaaattagcaggaagatggccagtaaaaacagtacatacagac
aatggcagcaatttcaccagtactacagttaaggccgcctgttggtgggcggggatcaag
caggaatttggcattccctacaatccccaaagtcaaggagtaatagaatctatgaataaa
gaattaaagaaaattataggacaggtaagagatcaggctgaacatcttaagacagcagta
caaatggcagtattcatccacaattttaaaagaaaaggggggattggggggtacagtgca
ggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaa
attacaaaaattcaaaattttcgggtttattacagggacagcagagatccagtttggaaa
ggaccagcaaagctcctctggaaaggtgaaggggcagtagtaatacaagataatagtgac
ataaaagtagtgccaagaagaaaagcaaagatcatcagggattatggaaaacagatggca
ggtgatgattgtgtggcaagtagacaggatgaggattaa

cPPT

5775 - 5790

RRE

6660 - 6893

Orf frame 1

6538 - 7686

HIV_Rev_NES

7416 - 7445

rb_glob_PA_terminator

7731 - 8160

M13_reverse_primer

8220 - 8202

M13_pUC_rev_primer

8241 - 8219

lac_promoter

8284 - 8255

SV40_origin

8425 - 8502

SV40_promoter

8390 - 8569

SV40pro_F_primer

8487 - 8506

SV40_PA_terminator

8583 - 8714

EBV_rev_primer

8671 - 8690

pBR322_origin

9557 - 8938

Orf frame 1

10572 - 9712

Ampicillin

10572 - 9712

AmpR_promoter

10642 - 10614

Unique restriction sites

SacII		1030
ClaI		1821
BclI		3419
EcoRV		3969
AflII		5733
SacI		6353
NheI		7718
StuI		8558
FspI		10007

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12260" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.