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pMIG-w
(Plasmid #12282)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12282 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    modified pMIG
  • Backbone size (bp) 7166
  • Modifications to backbone
    modification of pMIG vector to contain WRE element.
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use stbl3 cells from Invitrogen to decrease chance of recombination.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • IRES GFP (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Please acknowledge Dr. Yosef Refaeli (National Jewish Medical and Research Center) when using this plasmid in future publications.

pMIGw is a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus. The WRE element increases export to the cytoplasm and boosts protein expression by 2-10 fold.

IMPORTANT: This plasmid grows extremely slowly! Addgene recommends that when prepping DNA, you let your culture grow 18-20 hours (or more). Overnight will probably not be sufficient for a good prep.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIG-w was a gift from Luk Parijs (Addgene plasmid # 12282 ; http://n2t.net/addgene:12282 ; RRID:Addgene_12282)
  • For your References section:

    Interferon gamma is required for activation-induced death of T lymphocytes. Refaeli Y, Van Parijs L, Alexander SI, Abbas AK. J Exp Med. 2002 Oct 7. 196(7):999-1005. 10.1084/jem.20020666 PubMed 12370261