Purpose(Empty Backbone) a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12282||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonemodified pMIG
- Backbone size (bp) 7166
Modifications to backbonemodification of pMIG vector to contain WRE element.
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth instructionsUse stbl3 cells from Invitrogen to decrease chance of recombination.
Copy numberLow Copy
/ Fusion Protein
- IRES GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
Please acknowledge Dr. Yosef Refaeli (National Jewish Medical and Research Center) when using this plasmid in future publications.
pMIGw is a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus. The WRE element increases export to the cytoplasm and boosts protein expression by 2-10 fold.
IMPORTANT: This plasmid grows extremely slowly! Addgene recommends that when prepping DNA, you let your culture grow 18-20 hours (or more). Overnight will probably not be sufficient for a good prep.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIG-w was a gift from Luk Parijs (Addgene plasmid # 12282 ; http://n2t.net/addgene:12282 ; RRID:Addgene_12282)
For your References section:Interferon gamma is required for activation-induced death of T lymphocytes. Refaeli Y, Van Parijs L, Alexander SI, Abbas AK. J Exp Med. 2002 Oct 7. 196(7):999-1005. 10.1084/jem.20020666 PubMed 12370261
Map uploaded by the depositor.