PurposeBacterial expression plasmid for wild-type Dre with an N-terminal His-tag.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||123130||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5100
- Total vector size (bp) 6200
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameDre recombinase
Insert Size (bp)1029
- Promoter T7
/ Fusion Protein
- 6x His tag (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer GAAACAAGCGCTCATGAGCCCGAA
- 3′ sequencing primer GTCCCATTCGCCAATCCGGATATAG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-His-Dre was a gift from David Liu (Addgene plasmid # 123130 ; http://n2t.net/addgene:123130 ; RRID:Addgene_123130)
For your References section:High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases. Jeffrey L. Bessen, Lena K. Afeyan, Vlado Dančík, Luke W. Koblan, David B. Thompson, Chas Leichner, Paul A. Clemons & David R. Liu. Nature Communications volume 10, Article number: 1937 (2019) 10.1038/s41467-019-09987-0