Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases
Jeffrey L. Bessen, Lena K. Afeyan, Vlado Dančík, Luke W. Koblan, David B. Thompson, Chas Leichner, Paul A. Clemons & David R. Liu
Nature Communications volume 10, Article number: 1937 (2019)
Article

Plasmids from Article

ID Plasmid Purpose  
123128pET-His-CreBacterial expression plasmid for wild-type Cre with an N-terminal His-tag.
Loading...
123129pET-His-TreBacterial expression plasmid for Tre with an N-terminal His-tag.
Loading...
123130pET-His-DreBacterial expression plasmid for wild-type Dre with an N-terminal His-tag.
Loading...
123131pET-His-VCreBacterial expression plasmid for wild-type VCre with an N-terminal His-tag.
Loading...
123132pET-Bxb1-HisBacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.
Loading...
123133pCMV-CreMammalian expression vector for wild-type Cre.
Loading...
123134pCMV-TreMammalian expression vector for Tre.
Loading...
123135pCMV-Brec1Mammalian expression vector for Brec1.
Loading...
123136pCALNL-BsaI-EGFPGolden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.
Loading...
123137pBT100-neoGolden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.
Loading...