Addgene - pH3U3-Zif268 Plasmid Data

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12610: pH3U3-Zif268
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Plasmid 12610: pH3U3-Zif268

Gene/insert name:
Zif268 binding site in pH3U3
Insert size:
21
Vector backbone:
PH3U3-mcs
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert:
5821
Cloning site 5':
AscI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
CAAATATGTATCCGCTCATGAC List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
URA3, HIS3
Sequence: View sequences (2)
Principal Investigator:
Scot Wolfe
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Meng et al (Nat Biotechnol. 2005 Aug . 23(8):988-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12610" in your Materials and Methods section.