Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||12614||Bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneXL-1 blue F' episome
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameBacteria hybrid selection strain
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
This strain has a deletion in both the hisB and pyrF genes (the bacterial homologs of HIS3 and URA3) and it contains a F' episome bearing the lacl^q repressor.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:US0hisB-pyrF- was a gift from Scot Wolfe (Addgene plasmid # 12614)
For your References section:A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Meng X, Brodsky MH, Wolfe SA. Nat Biotechnol. 2005 Aug . 23(8):988-94. 10.1038/nbt1120 PubMed 16041365