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12614: US0hisB-pyrF…
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Browse > Scot Wolfe > Meng et al > US0hisB-pyrF-
Bacterial strain 12614: US0hisB-pyrF-

  • Bacteria hybrid selection strain

  • XL-1 blue F' episome
    (Search Vector Database)

  • Bacterial Expression

  • N/A List of Sequencing Primers

  • Tetracycline

  • DH5alpha

  • 37

  • Low Copy

  • Scot Wolfe

  • MTA

Comments: 

This strain has a deletion in both the hisB and pyrF genes (the bacterial homologs of HIS3 and URA3) and it contains a F' episome bearing the lacl^q repressor.

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Meng et al (Nat Biotechnol. 2005 Aug . 23(8):988-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12614" in your Materials and Methods section.