Addgene - pB1H2 Plasmid Data

Plasmid 12613: pB1H2

Gene/insert name:
dorsal RHR in pB1H2
Alt name:
dorsal
Insert size:
887
Species:
D. melanogaster (fly)
GenBank ID:
CG6667
Entrez Gene:
dl (Dmel_CG6667, CG6667, DL, Dl, Dmel\CG6667, GSd447, anon-EST:GressD7, d, dL, fs(2)k10816, mat(2)dorsal)
Fusion protein or tag:
flag
Terminal:
N terminal on backbone
Vector backbone:
pB1H2
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4093
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
AvrII
Site destroyed during cloning:
No
5' sequencing primer:
CGTGATGTACGTCAGCCTGAAG List of Sequencing Primers
3' sequencing primer:
TTGTCGGCCTTTTTCTAGTCTCTAGA
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Scot Wolfe
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Meng et al (Nat Biotechnol. 2005 Aug . 23(8):988-94. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12613" in your Materials and Methods section.

Price: $65.00

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