|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||126475||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 4111
Modifications to backboneThe promoter, multiple cloning site and termination regions have been replaced with the bidirectional expression cassette of the pBI-CMV1 vector (Clontech)
Vector typeMammalian Expression
- Promoter CMV
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer GGGGTCATTAGTTCATAGCCCATAT
- 3′ sequencing primer CAAAACCGCATCACCATGGTAATAG (Common Sequencing Primers)
Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-BI was a gift from Bill Hansson (Addgene plasmid # 126475 ; http://n2t.net/addgene:126475 ; RRID:Addgene_126475)
For your References section:Optimization of insect odorant receptor trafficking and functional expression via transient transfection in HEK293 cells. Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, Lavista-Llanos S. bioRxiv 669127 10.1101/669127