Addgene - CAG pShuttle Plasmid Data

Gene/insert name:
None
Vector backbone:
pShuttle
(Search Vector Database)
Vector type:
Mammalian Expression, Adenoviral
Modifications to Backbone:
CAG promoter inserted into pShuttle (CAG promoter = a combination of chicken beta-actin promoter and cytomegalovirus immediate-early enhancer)
5' sequencing primer:
pCAG-F List of Sequencing Primers
Bacterial resistance:
Kanamycin
Growth strain:
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Ronald Kahn
Terms and Licenses:
MTA

Comments:

pShuttle is used to make adenovirus in the AdEasy system. The CAG promoter was inserted for stronger expression and a MCS compatible with pBS.

See author's map for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13427" in your Materials and Methods section.