Addgene - pCAG-Cre:GFP Plasmid Data

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Plasmid 13776: pCAG-Cre:GFP

Gene/insert name:		Cre-GFP fusion protein
Alternative names:		Cre recombinase
Insert size (bp):		1784
Species of gene(s):		Bacteriophage P1
Fusion proteins or tags:		GFP
Terminal:		C terminal on insert
Vector backbone:		pCAGEN (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		4779
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		NotI
Site destroyed during cloning:		No
5' Sequencing primer:		pCAG-F (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		The coding sequence of Cre was amplified by PCR using pxCANCre (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo) as a template. GFP was from pEGFP-N1 (Clontech).
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Connie Cepko
Terms and Licenses:		MTA

Comments: Kozak consensus sequence was added before the start ATG. GFP-tag was added at the C-terminus of Cre. Cre-GFP fusion protein is localized in the cell nucleus.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

CAG_enhancer

84 - 371

Orf frame 1

1533 - 802

pCAG_F_primer

1678 - 1697

CRE_NLS

1749 - 2744

Orf frame 3

1737 - 3500

EGFP_N_primer

2847 - 2826

EGFP

2781 - 3497

Orf frame 3

3638 - 2649

EGFP_C_primer

3434 - 3455

rb_glob_PA_terminator

3519 - 4020

M13_reverse_primer

4080 - 4062

M13_pUC_rev_primer

4101 - 4079

lac_promoter

4144 - 4115

SV40_origin

4285 - 4362

SV40_promoter

4250 - 4429

SV40pro_F_primer

4347 - 4366

SV40_PA_terminator

4443 - 4574

EBV_rev_primer

4531 - 4550

pBR322_origin

5417 - 4798

Orf frame 1

6432 - 5572

Ampicillin

6432 - 5572

AmpR_promoter

6502 - 6474

Unique restriction sites

SalI		1
SpeI		18
ApaI		863
SacII		1030
XbaI		1623
EcoRI		1719
NruI		1951
BstBI		2146
EcoRV		2288
ClaI		2543
NotI		3503
BglII		3584
PstI		4043
HindIII		4048
StuI		4418
FspI		5867

Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T et al. (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13776" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.