Addgene - pTV-Nanog Bgeo Plasmid Data

Plasmid 13837: pTV-Nanog Bgeo

Gene/insert name:
IRES-Bgeo-pA cassette flanked by Nanog homologous regions
Alt name:
Nanog
Insert size:
11000
Species:
M. musculus (mouse)
Entrez Gene:
Nanog (2410002E02Rik, ENK, ecat4)
Mutation:
None
Vector backbone:
pBluescript SK
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mammalian Expression ; Beta-gal
Backbone size w/o insert (bp):
3000
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T3 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Mitsui et al (Cell. 2003 May 30. 113(5):631-42. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13837" in your Materials and Methods section.