Addgene - pUASTP2.1 Plasmid Data

Plasmid 13842: pUASTP2.1

Gene/insert name:
mini-white
Alt name:
white
Insert size:
4000
Species:
D. melanogaster (fly)
Entrez Gene:
w (Dmel_CG2759, BACN33B1.1, CG2759, DMWHITE, Dmel\CG2759, EG:BACN33B1.1, Hid, W, c23, e(g), m(g), mw, w(AT)[[13]])
Vector backbone:
pUAST
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
4300
Cloning method:
Gateway Cloning
Cloning site 5':
attP
Site destroyed during cloning:
No
Cloning site 3':
attP
Site destroyed during cloning:
No
5' sequencing primer:
Pry1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
attP from Michele Calos, Stanford
Sequence: View sequences (2)
Principal Investigator:
Ting Wu
Terms and Licenses:
MTA

Comments:

pUASTP2.1 contains a P-element target for RMCE, the mini-white gene flanked by inverted attP sites. It is a derivative of PUASTP2 in which the UAS and TATA sequences flanking the cassette have been removed. (Specifically, PUASTP2 was digested with BamHI and StuI to remove UAS-TATA-attP, and an attP-containing BamHI-EcoRV fragment from pTA-attP was inserted). This vector can be used to generate target lines by standard P element transgenesis.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. Bateman et al (Genetics. 2006 Jun . 173(2):769-77. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13842" in your Materials and Methods section.

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