pLenti-lox-GFP shRNA p19-2
(Plasmid #14091)

Loading...
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLL3.7
  • Backbone manufacturer
    Available from Addgene (#11795)
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    p19 ARF shRNA -2
  • Alt name
    p19ARF
  • Alt name
    ARF-INK4a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    50
  • Entrez Gene
    Cdkn2a (a.k.a. RP23-166I8.5, ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer H1
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

p19 ARF shRNA expressed from a lentiviral vector. The p19 ARF shRNA together with the H1 promoter was cloned from pSIRIP-puro into pLL3.7. Therefore, although the shRNA is normally expressed from the U6 promoter of pLL3.7, p19-ARF shRNA is expressed here from the H1 promoter.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-lox-GFP shRNA p19-2 was a gift from Tyler Jacks (Addgene plasmid # 14091)
  • For your References section:

    Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry. Sage J, Miller AL, Perez-Mancera PA, Wysocki JM, Jacks T. Nature. 2003 Jul 10. 424(6945):223-8. 10.1038/nature01764 PubMed 12853964