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Plasmid 14091: pLenti-lox-GFP shRNA p19-2
  • p19 ARF shRNA -2

  • p19ARF

  • ARF-INK4a

  • 50

  • M. musculus (mouse)

  • Cdkn2a (RP23-166I8.5, ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF)

  • pLL3.7
    (Search Vector Database)

  • Available from Addgene (#11795)

  • Mammalian Expression, Lentiviral, RNAi, Cre/Lox

  • 8000

  • BglII

  • Unknown

  • XhoI

  • Unknown

  • H1 List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • View sequences (1)
  • Tyler Jacks

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

p19 ARF shRNA expressed from a lentiviral vector. The p19 ARF shRNA together with the H1 promoter was cloned from pSIRIP-puro into pLL3.7. Therefore, although the shRNA is normally expressed from the U6 promoter of pLL3.7, p19-ARF shRNA is expressed here from the H1 promoter.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry. Sage et al (Nature. 2003 Jul 10. 424(6945):223-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14091" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only