Addgene - pLenti-lox-GFP shRNA p19-2 Plasmid Data

Plasmid 14091: pLenti-lox-GFP shRNA p19-2

Gene/insert name:
p19 ARF shRNA -2
Alt name:
p19ARF
Alt name:
ARF-INK4a
Insert size:
50
Species:
M. musculus (mouse)
Entrez Gene:
Cdkn2a (RP23-166I8.5, ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF)
Vector backbone:
pLL3.7
(Search Vector Database)
Backbone manufacturer:
Available from Addgene (#11795)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
8000
Cloning site 5':
BglII
Site destroyed during cloning:
Unknown
Cloning site 3':
XhoI
Site destroyed during cloning:
Unknown
5' sequencing primer:
H1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

p19 ARF shRNA expressed from a lentiviral vector. The p19 ARF shRNA together with the H1 promoter was cloned from pSIRIP-puro into pLL3.7. Therefore, although the shRNA is normally expressed from the U6 promoter of pLL3.7, p19-ARF shRNA is expressed here from the H1 promoter.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry. Sage et al (Nature. 2003 Jul 10. 424(6945):223-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14091" in your Materials and Methods section.