|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14782||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSicoR PGK puro
- Backbone size w/o insert (bp) 7700
Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameLuciferase shRNA
- Cloning method Restriction Enzyme
- 5′ sequencing primer mU6-F (Common Sequencing Primers)
shluc: 5’-GTCCA GATTG TCCGC AACTA-3’
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSicoR shluc was a gift from Tyler Jacks (Addgene plasmid # 14782 ; http://n2t.net/addgene:14782 ; RRID:Addgene_14782)
For your References section:Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T. Nat Genet. 2007 Apr 1. ():. 10.1038/ng2003 PubMed 17401365