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pTRE-TIGHT miR-1 (aka:pTRE-m1d)
(Plasmid #14896)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14896 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTRE-TIGHT
  • Backbone manufacturer
    Bartel Lab
  • Backbone size w/o insert (bp) 2600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    miR-1
  • Alt name
    miR-1d
  • Alt name
    miR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site ClaI (unknown if destroyed)
  • 5′ sequencing primer pLNCX primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Expression of a transcript behind TRE-Tight tetracycline regulatable promoter. This vector is part of the BD-Tet-ON/Tet-Off system for inducible expression in mammalian cells.

miR-1 is predicted to target Glucose-6 phosphate 1-dehydrogenase (G6PD).

The sequence provided is approximated based on the information that miR-1 was inserted at the BamHI and ClaI sites of pTRE-TIGHT. There may be slight differences in the actual sequence near the cloning sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE-TIGHT miR-1 (aka:pTRE-m1d) was a gift from David Bartel (Addgene plasmid # 14896 ; http://n2t.net/addgene:14896 ; RRID:Addgene_14896)
  • For your References section:

    Prediction of mammalian microRNA targets. Lewis BP, Shih IH, Jones-Rhoades MW, Bartel DP, Burge CB. Cell. 2003 Dec 26. 115(7):787-98. 10.1016/S0092-8674(03)01018-3 PubMed 14697198