|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14937||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneFOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Flag FoxO3 was a gift from Joan Massague (Addgene plasmid # 14937 ; http://n2t.net/addgene:14937 ; RRID:Addgene_14937)
For your References section:Integration of Smad and forkhead pathways in the control of neuroepithelial and glioblastoma cell proliferation. Seoane J, Le HV, Shen L, Anderson SA, Massague J. Cell. 2004 Apr 16. 117(2):211-23. 10.1016/S0092-8674(04)00298-3 PubMed 15084259
Map uploaded by the depositor.