Addgene - Flag-mPIAS1 Plasmid Data

Plasmid 15206: Flag-mPIAS1

Gene/insert name:
PIAS1
Species:
M. musculus (mouse)
Entrez Gene:
Pias1 (2900068C24Rik, Ddxbp1, GBP)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
pCMV-Flag
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3800
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
hGHpA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Ke Shuai
Terms and Licenses:
MTA

Comments:

BglII/XhoI insert cloned into BglII/SalI sites of vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Inhibition of Stat1-mediated gene activation by PIAS1. Liu et al (Proc Natl Acad Sci U S A. 1998 Sep 1. 95(18):10626-31. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15206" in your Materials and Methods section.