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Flag-mPIAS3
(Plasmid #15207)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15207 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIAS3
  • Species
    M. musculus (mouse)
  • Mutation
    V580I
  • GenBank ID
    AAB88902.2
  • Entrez Gene
    Pias3 (a.k.a. Pias3l)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Please note that Addgene's sequencing results identified a V580I difference when compared to GenBank reference sequence AAB88902.2. The depositing laboratory has confirmed that plasmid this is exact construct used in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-mPIAS3 was a gift from Ke Shuai (Addgene plasmid # 15207 ; http://n2t.net/addgene:15207 ; RRID:Addgene_15207)
  • For your References section:

    Specific inhibition of Stat3 signal transduction by PIAS3. Chung CD, Liao J, Liu B, Rao X, Jay P, Berta P, Shuai K. Science. 1997 Dec 5. 278(5344):1803-5. 10.1126/science.278.5344.1803 PubMed 9388184