Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Flag-hPIASy
(Plasmid #15208)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15208 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIASy
  • Species
    H. sapiens (human)
  • Entrez Gene
    PIAS4 (a.k.a. PIAS-gamma, PIASY, Piasg, ZMIZ6)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BglII/XhoI insert cloned into BamHI/SalI sites of vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-hPIASy was a gift from Ke Shuai (Addgene plasmid # 15208 ; http://n2t.net/addgene:15208 ; RRID:Addgene_15208)
  • For your References section:

    A transcriptional corepressor of Stat1 with an essential LXXLL signature motif. Liu B, Gross M, ten Hoeve J, Shuai K. Proc Natl Acad Sci U S A. 2001 Mar 13. 98(6):3203-7. 10.1073/pnas.051489598 PubMed 11248056