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pMT-EGFP-Actin5c
(Plasmid #15312)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15312 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMT-V5/His'B'
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Actin5c
  • Alt name
    Actin 5c
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1871
  • Entrez Gene
    Act5C (a.k.a. Dmel_CG4027, A, A4V404_DROME, ACT, ACT1_DROME, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, ArpA, BAP47, Bap47, CG4027, Dmel\CG4027, F-actin, G-actin, M32055, T11, act, act 5C, act5C, actin, actin5C, anon-EST:fe2D2, beta-actin, beta-actin/Bap47, chrX:5748184..5748304, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486)
  • Tags / Fusion Proteins
    • EGFP (N terminal on insert)
    • His (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-EGFP-Actin5c was a gift from Ron Vale (Addgene plasmid # 15312 ; http://n2t.net/addgene:15312 ; RRID:Addgene_15312)
  • For your References section:

    Molecular requirements for actin-based lamella formation in Drosophila S2 cells. Rogers SL, Wiedemann U, Stuurman N, Vale RD. J Cell Biol. 2003 Sep 15. 162(6):1079-88. 10.1083/jcb.200303023 PubMed 12975351