Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16048||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepGL2-basic vector (BV)
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameId1 promoter region
SpeciesM. musculus (mouse)
Insert Size (bp)1673
Mutation-1585 to +88 of promoter
Entrez GeneId1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:1.5 BV was a gift from Robert Benezra (Addgene plasmid # 16048 ; http://n2t.net/addgene:16048 ; RRID:Addgene_16048)
For your References section:Transcription of the dominant-negative helix-loop-helix protein Id1 is regulated by a protein complex containing the immediate-early response gene Egr-1. Tournay O, Benezra R. Mol Cell Biol. 1996 May . 16(5):2418-30. PubMed 8628310