Vector backboneMDH1-PGK-GFP 2.0
- Backbone size w/o insert (bp) 6963
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
SpeciesH. sapiens (human)
Insert Size (bp)315
Entrez GeneMIR10B (a.k.a. MIRN10B, hsa-mir-10b, miRNA10B, mir-10b)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer ggatcccaatatttgcatgtcgc (Common Sequencing Primers)
Terms and Licenses
The miRNA gene was PCR-amplified from normal human genomic DNA and cloned into the MDH1-PGK-GFP 2.0 retroviral vector. It includes miR-10b stem-loop and ~100bp flanking sequences on both sides.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MDH1-PGK-GFP microRNA-10b was a gift from Bob Weinberg (Addgene plasmid # 16070)
For your References section:Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Ma L, Teruya-Feldstein J, Weinberg RA. Nature. 2007 Oct 11. 449(7163):682-8. 10.1038/nature06174 PubMed 17898713
Map generated by Addgene from plasmid data.