Addgene - pMIR-10b Plasmid Data

Plasmid 19118: pMIR-10b

Gene/insert name:
microRNA 10b
Alt name:
MIRN10B
Insert size:
35
Species:
H. sapiens (human)
Entrez Gene:
MIR10B (MIRN10B, hsa-mir-10b, miRNA10B, mir-10b)
Vector backbone:
pMIR-report
(Search Vector Database)
Backbone manufacturer:
Ambion
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6470
Cloning site 5':
SpeI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
Luc-F List of Sequencing Primers
3' sequencing primer:
M13-F20
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Human mir-10b binding site (the exact reverse complementary sequence of mir-10b) was generated by annealing of 2 oligos, as described in the reference.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Ma et al (Nature. 2007 Oct 11. 449(7163):682-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19118" in your Materials and Methods section.