BDS-2 3x MUT (p53 binding site)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16516||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 4900
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namep53 binding site
Alt name14-3-3 sigma promoter
SpeciesH. sapiens (human)
MutationMutant p53 binding sites
Entrez GeneSFN (a.k.a. YWHAS)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
This reporter contains 3 copies of the mutant p53-binding site from the 14-3-3 sigma promoter. The following oligonucleotide pairs were used: 5'-CCTGTAGAAT TATCCCAGAA ATTTCCCTAC TCCGTAC-3' and 5'-GGAGTAGGGA AATTTCTGGG ATAATTCTAC AGGGTAC-3' for BDS-2*, altered at critical p53-binding residues.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BDS-2 3x MUT (p53 binding site) was a gift from Bert Vogelstein (Addgene plasmid # 16516 ; http://n2t.net/addgene:16516 ; RRID:Addgene_16516)
For your References section:14-3-3 sigma is a p53-regulated inhibitor of G2/M progression. Hermeking H, Lengauer C, Polyak K, He TC, Zhang L, Thiagalingam S, Kinzler KW, Vogelstein B. Mol Cell. 1997 Dec . 1(1):3-11. 10.1016/S1097-2765(00)80002-7 PubMed 9659898
Map uploaded by the depositor.