Epac-S-H74
(Plasmid
#170338)
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PurposemTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170338 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST)
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SpeciesH. sapiens (human)
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MutationDeletion of DEP domain, Catalytically dead T781A & F782A
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Entrez GeneRAPGEF3 (a.k.a. CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Epac-S-H74 was a gift from Kees Jalink (Addgene plasmid # 170338 ; http://n2t.net/addgene:170338 ; RRID:Addgene_170338) -
For your References section:
A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range. Klarenbeek JB, Goedhart J, Hink MA, Gadella TW, Jalink K. PLoS One. 2011 Apr 29;6(4):e19170. doi: 10.1371/journal.pone.0019170. 10.1371/journal.pone.0019170 PubMed 21559477
