ZE10 sonic hedgehog pT7TS
(Plasmid #17059)

• Depositing Lab: Randall Moon
• Publication: Ekker et al Curr Biol. 1995 Aug 1. 5(8):944-55.

Backbone

• Vector backbone: pT7TS
• Backbone size w/o insert (bp): 3000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: sonic hedgehog
• Species: D. rerio (zebrafish)
• Entrez Gene: shha (a.k.a. ShhNC, fc83d08, shh, syu, vhh-1, vhh1, wu:fc83d08)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (unknown if destroyed)
• 3′ cloning site: SpeI (unknown if destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Steve Ekker clone E, in vector pT7TS. Linearize with BamH1 and transcribe with T7 for sense RNA for injections.
Citation: Ekker et al. (1995), Current Biology 5:944-955.

Made by insertion of shh open-reading frame into the SpeI site of the pT7TS vector. The ORF was made by PCR using the following primers: shhA (5′-GGACTAG-TCACCATGCGGCTTTTGACGAGA-3′) and shhB (5′-AGCATACTAGTAGTCTCAGCTTGAGTTTACTGA-3′).