ZE10 sonic hedgehog pT7TS
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17059||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
Gene/Insert namesonic hedgehog
SpeciesD. rerio (zebrafish)
Entrez Geneshha (a.k.a. ShhNC, fc83d08, shh, syu, vhh-1, vhh1, wu:fc83d08)
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (unknown if destroyed)
- 3′ cloning site SpeI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Steve Ekker clone E, in vector pT7TS. Linearize with BamH1 and transcribe with T7 for sense RNA for injections.
Citation: Ekker et al. (1995), Current Biology 5:944-955.
Made by insertion of shh open-reading frame into the SpeI site of the pT7TS vector. The ORF was made by PCR using the following primers: shhA (5′-GGACTAG-TCACCATGCGGCTTTTGACGAGA-3′) and shhB (5′-AGCATACTAGTAGTCTCAGCTTGAGTTTACTGA-3′).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE10 sonic hedgehog pT7TS was a gift from Randall Moon (Addgene plasmid # 17059 ; http://n2t.net/addgene:17059 ; RRID:Addgene_17059)