|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17223||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4842
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePolII promoter
SpeciesM. musculus (mouse)
Insert Size (bp)710
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (destroyed during cloning)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer pGV-2U (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPolII-Luc was a gift from Shinya Yamanaka (Addgene plasmid # 17223 ; http://n2t.net/addgene:17223 ; RRID:Addgene_17223)
For your References section:Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Cell. 2007 Nov 30. 131(5):861-72. 10.1016/j.cell.2007.11.019 PubMed 18035408
Map uploaded by the depositor.