Addgene - pPolII-Luc Plasmid Data

Plasmid 17223: pPolII-Luc

Gene/insert name:
PolII promoter
Insert size:
710
Species:
M. musculus (mouse)
Mutation:
None
Vector backbone:
pGV-BM2
(Search Vector Database)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4842
Cloning site 5':
KpnI
Site destroyed during cloning:
Yes
Cloning site 3':
NheI
Site destroyed during cloning:
No
5' sequencing primer:
pGV-2U List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi et al (Cell. 2007 Nov 30. 131(5):861-72. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17223" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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