Addgene - Rosa26 mT/mG Plasmid Data

Plasmid 17787: Rosa26 mT/mG

Gene/insert name:
Rosa26 mT/mG
Alt name:
TdTomato
Alt name:
mT
Alt name:
EGFP
Insert size:
5500
Species:
Aquorea victoria
Fusion protein or tag:
membrane tag
Terminal:
N terminal on insert
Vector backbone:
pBluescript
(Search Vector Database)
Vector type:
Mammalian Expression, Mouse Targeting, Cre/Lox
Backbone size w/o insert (bp):
4969
Cloning site 5':
See map
Site destroyed during cloning:
No
Cloning site 3':
See map
Site destroyed during cloning:
No
5' sequencing primer:
See map List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (4)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Liqun Luo
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

See the 'Notes' section for the annotated GenBank file. Please note that some portions of the sequence (e.g., Rosa26 targeting arms and CA promoter) may not be entirely accurate.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A global double-fluorescent Cre reporter mouse. Muzumdar et al (Genesis. 2007 Sep . 45(9):593-605. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17787" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

13777: pCAG-ERT2CreERT2