|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1782||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCI neo
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Alt nameEver Shorter Telomeres 2
Alt nametelomerase reverse transcriptase
SpeciesH. sapiens (human)
Insert Size (bp)3510
MutationClone is catalytically active but is dead in vivo.
Entrez GeneTERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCI neo-hEST2-HA was a gift from Bob Weinberg (Addgene plasmid # 1782 ; http://n2t.net/addgene:1782 ; RRID:Addgene_1782)
For your References section:hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddle SD, Ziaugra L, Beijersbergen RL, Davidoff MJ, Liu Q, Bacchetti S, Haber DA, Weinberg RA. Cell 1997 Aug 22;90(4):785-95. 10.1016/S0092-8674(00)80538-3 PubMed 9288757
Map uploaded by the depositor.