Addgene - pCI neo-hEST2-HA Plasmid Data

Plasmid 1782: pCI neo-hEST2-HA

Gene/insert name:
hTERT
Alt name:
Ever Shorter Telomeres 2
Alt name:
telomerase reverse transcriptase
Alt name:
hTERT-HA
Insert size:
3510
Species:
H. sapiens (human)
GenBank ID:
AF018167
Entrez Gene:
TERT (CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Mutation:
Clone is catalytically active but is dead in vivo.
Vector backbone:
pCI neo
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
EBV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Meyerson et al (Cell 1997 Aug 22;90(4):785-95. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1782" in your Materials and Methods section.