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pCI neo-hEST2-HA
(Plasmid #1782)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCI neo
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    hTERT
  • Alt name
    Ever Shorter Telomeres 2
  • Alt name
    telomerase reverse transcriptase
  • Alt name
    hTERT-HA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3510
  • Mutation
    Clone is catalytically active but is dead in vivo.
  • GenBank ID
    AF018167
  • Entrez Gene
    TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI neo-hEST2-HA was a gift from Bob Weinberg (Addgene plasmid # 1782)
  • For your References section:

    hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddle SD, Ziaugra L, Beijersbergen RL, Davidoff MJ, Liu Q, Bacchetti S, Haber DA, Weinberg RA. Cell 1997 Aug 22;90(4):785-95. 10.1016/S0092-8674(00)80538-3 PubMed 9288757