USO hisB- pyrF- rpoZ-
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||18049||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneXL-1 blue F' episome
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameomega knockout Bacteria hybrid selection strain
Insert Size (bp)450
MutationThis is the US0 selection strain that has hisB, pyrF and rpoZ knocked out. A zeocin cassette has been knocked in to the rpoZ locus
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
This strain has a deletion in hisB, pyrF and ropZ genes (the bacterial homologs of HIS3 and URA3 and the omega subunit) and it contains a F' episome bearing the lacl^q repressor.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:USO hisB- pyrF- rpoZ- was a gift from Scot Wolfe (Addgene plasmid # 18049)
For your References section:A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA. Nucleic Acids Res. 2008 Mar 10. ():. 10.1093/nar/gkn048 PubMed 18332042