Addgene - USO hisB- pyrF- rpoZ- Plasmid Data

Bacterial strain 18049: USO hisB- pyrF- rpoZ-

Gene/insert name:
omega knockout Bacteria hybrid selection strain
Insert size:
450
Mutation:
This is the US0 selection strain that has hisB, pyrF and rpoZ knocked out. A zeocin cassette has been knocked in to the rpoZ locus
Vector backbone:
XL-1 blue F' episome
(Search Vector Database)
Vector type:
Bacterial Expression
5' sequencing primer:
N/A List of Sequencing Primers
Bacterial resistance(s)
Tetracycline
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
Zeocin
Principal Investigator:
Scot Wolfe
Terms and Licenses:
MTA

Comments:

This strain has a deletion in hisB, pyrF and ropZ genes (the bacterial homologs of HIS3 and URA3 and the omega subunit) and it contains a F' episome bearing the lacl^q repressor.

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes et al (Nucleic Acids Res. 2008 Mar 10. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18049" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

18045: pB1H2w2-zif268

12609: pH3U3-mcs

18046: pH3U3-zif268 (omega)

18039: pB1H2w5-Prd

18044: pB1H2w2-mutOdd