|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18046||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5807
Vector typeBacterial Expression
Selectable markersURA3, HIS3
Growth in Bacteria
Copy numberLow Copy
Gene/Insert namezif268 binding site
Insert Size (bp)15
MutationThe preferred binding site for zif268 is inserted 10bp upstream of the -35box. This reporter plasmid is to be used as a positive test with pB1H2w2-zif268.
- Cloning method Restriction Enzyme
- 5′ cloning site Not1 (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer HU100 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pH3U3-zif268 (omega) was a gift from Scot Wolfe (Addgene plasmid # 18046 ; http://n2t.net/addgene:18046 ; RRID:Addgene_18046)
For your References section:A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA. Nucleic Acids Res. 2008 Mar 10. ():. 10.1093/nar/gkn048 PubMed 18332042