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pH3U3-zif268 (omega)
(Plasmid #18046)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18046 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pH3U3
  • Backbone size w/o insert (bp) 5807
  • Vector type
    Bacterial Expression
  • Selectable markers
    URA3, HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    zif268 binding site
  • Insert Size (bp)
    15
  • Mutation
    The preferred binding site for zif268 is inserted 10bp upstream of the -35box. This reporter plasmid is to be used as a positive test with pB1H2w2-zif268.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer HU100
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH3U3-zif268 (omega) was a gift from Scot Wolfe (Addgene plasmid # 18046 ; http://n2t.net/addgene:18046 ; RRID:Addgene_18046)
  • For your References section:

    A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA. Nucleic Acids Res. 2008 Mar 10. ():. 10.1093/nar/gkn048 PubMed 18332042