|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18045||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3700
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)267
MutationZif268 is fused to the omega subunit of RNA polymerase and is to be used as a positive control.
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site Xba1 (destroyed during cloning)
- 5′ sequencing primer OMG5 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pB1H2w2-zif268 was a gift from Scot Wolfe (Addgene plasmid # 18045 ; http://n2t.net/addgene:18045 ; RRID:Addgene_18045)
For your References section:A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA. Nucleic Acids Res. 2008 Mar 10. ():. 10.1093/nar/gkn048 PubMed 18332042