|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18044||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3633
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemutant Odd Skipped
SpeciesD. melanogaster (fly)
Insert Size (bp)422
MutationA mutant version of the zinc finger protein Odd Skipped, which is inactivated by a frame shift mutation, is fused to the omega subunit. To be used as a negative control.
- Cloning method Restriction Enzyme
- 5′ cloning site Not1 (not destroyed)
- 3′ cloning site Xba1 (not destroyed)
- 5′ sequencing primer OMG5 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pB1H2w2-mutOdd was a gift from Scot Wolfe (Addgene plasmid # 18044 ; http://n2t.net/addgene:18044 ; RRID:Addgene_18044)
For your References section:A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA. Nucleic Acids Res. 2008 Mar 10. ():. 10.1093/nar/gkn048 PubMed 18332042