Plasmid 1836: pRET.IL.IRES-EGFP (No HSV-TK)

• Gene/insert name: None
• Alt name: retroviral RET construct
• Alt name: removable exon trap
• Alt name: Type I vector
• Vector backbone: pRET
  (Search Vector Database)
• Vector type: Mammalian Expression, Retroviral, Cre/Lox
• 5' sequencing primer: na
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Selectable markers: Neomycin
• Sequence: View sequences (1)
• Map: View map
• Principal Investigator: Philip Leder
• Terms and Licenses: MTA

Comments: 

See scanned map (#4).
The HSV-TK cassette is eliminated from this vector. Virus production should be fine, but you can not titrate the virus accurately because of the lack of the HSV-TK cassette, the only constitutive marker in RET vectors. You can easily excise all the essential components as an XbaI-XbaI fragment. To obtain larger numbers of G418-resistant clones, this vector carries a strong NEO promoter. This RET vector would be the first choice for making gene-disrupted mice by using transfected or infected ES cells. (Leder #C-1023)

Article: RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells. Ishida et al (Nucleic Acids Res 1999 Dec 15;27(24):e35. PubMed)