Plasmid 1838: pRET.IIS.IRES-EGFP

• Gene/insert name: None
• Alt name: retroviral RET construct
• Alt name: removable exon trap
• Alt name: Type II vector
• Vector backbone: pRET
  (Search Vector Database)
• Vector type: Mammalian Expression, Retroviral, Cre/Lox
• 5' sequencing primer: na
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Selectable markers: Neomycin
• Sequence: View sequences (1)
• Map: View map
• Principal Investigator: Philip Leder
• Terms and Licenses: MTA

Comments: 

See scanned map (#3).
This is a modified RET-EGFP vector for reasonably high-titer virus production, up to ~4x10^5 cfu/ml. However, the selectivity for the intragenic integration would not be as high as that achieved by other RET vectors (Type I). An additional XbaI site between EGFP and GHpA prevents isolation of essential elements of the vector on a single XbaI-XbaI fragment; this vector is not suitable for transfection.
(Leder #C-1022)

Article: RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells. Ishida et al (Nucleic Acids Res 1999 Dec 15;27(24):e35. PubMed)