- Backbone size w/o insert (bp) 6800
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
Insert Size (bp)700
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI, SfiI, or AgeI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
See author's map for more information. CAG–GFP uses the backbone of pCLNCX v.2, and the expression of GFP is driven by the compound promoter CAG. This vector also contains the woodchuck hepatitis virus posttranscriptional element, which reportedly increases protein production by stabilizing the transcript.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CAG-GFP was a gift from Fred Gage (Addgene plasmid # 16664)
For your References section:Distinct morphological stages of dentate granule neuron maturation in the adult mouse hippocampus. Zhao C, Teng EM, Summers RG, Ming GL, Gage FH. J Neurosci. 2006 Jan 4. 26(1):3-11. 10.1523/JNEUROSCI.3648-05.2006 PubMed 16399667
Map uploaded by the depositor.