CAG-GFP
(Plasmid #16664)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    CAG-GFP
  • Backbone size w/o insert (bp) 6800
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    eGFP
  • Insert Size (bp)
    700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI, SfiI, or AgeI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

See author's map for more information. CAG–GFP uses the backbone of pCLNCX v.2, and the expression of GFP is driven by the compound promoter CAG. This vector also contains the woodchuck hepatitis virus posttranscriptional element, which reportedly increases protein production by stabilizing the transcript.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG-GFP was a gift from Fred Gage (Addgene plasmid # 16664)
  • For your References section:

    Distinct morphological stages of dentate granule neuron maturation in the adult mouse hippocampus. Zhao C, Teng EM, Summers RG, Ming GL, Gage FH. J Neurosci. 2006 Jan 4. 26(1):3-11. 10.1523/JNEUROSCI.3648-05.2006 PubMed 16399667