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CAG-GFP/cre
(Plasmid #49054)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49054 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    CAG-GFP
  • Backbone manufacturer
    Gage lab (Addgene plasmid# 16664)
  • Backbone size w/o insert (bp) 6817
  • Total vector size (bp) 8681
  • Modifications to backbone
    vector backbone based on the pCL system by Naviaux et al.,J. Virol. 70, 5701–5705 (1996).
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP/Cre
  • Alt name
    GFP-fused Cre recombinase
  • Species
    Synthetic
  • Insert Size (bp)
    1870
  • GenBank ID
  • Promoter CAG

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CTC GGC AAC GTG CTG GTT GTT
  • 3′ sequencing primer CAACATAGTTAAGAATACCAGTC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG-GFP/cre was a gift from Fred Gage (Addgene plasmid # 49054 ; http://n2t.net/addgene:49054 ; RRID:Addgene_49054)
  • For your References section:

    Retrovirus-mediated single-cell gene knockout technique in adult newborn neurons in vivo. Tashiro A, Zhao C, Gage FH. Nat Protoc. 2006;1(6):3049-55. 10.1038/nprot.2006.473 PubMed 17406567