Addgene - pMXs-gw Plasmid Data

Gene/insert name:
Gateway cassette A
Alt name:
attR
Insert size:
1750
Vector backbone:
pMXs
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert:
4750
Cloning method:
Gateway Cloning
Cloning site 5':
EcoRI
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
Yes
5' sequencing primer:
pMX-S1811 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
ccdB Survival
Growth temperature (℃):
37
Growth instructions:
DB3.1
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway cassette is from Invitrogen Corporation.
Sequence: View sequences (1)
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Comments:

The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi et al (Cell. 2006 Aug 25. 126(4):663-76. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18656" in your Materials and Methods section.