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pGL3B-1081
(Plasmid #18759)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18759 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    thioredoxin-interacting protein promoter
  • Alt name
    txnip
  • Alt name
    VDUP-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1128
  • Entrez Gene
    Txnip (a.k.a. 1200008J08Rik, Hyplip1, THIF, Tbp-2, VDUP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer TAGCAAAATA GGCTGTCCC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3B-1081 was a gift from Clark Distelhorst (Addgene plasmid # 18759 ; http://n2t.net/addgene:18759 ; RRID:Addgene_18759)
  • For your References section:

    Thioredoxin-interacting protein (txnip) is a glucocorticoid-regulated primary response gene involved in mediating glucocorticoid-induced apoptosis. Wang Z, Rong YP, Malone MH, Davis MC, Zhong F, Distelhorst CW. Oncogene. 2006 Mar 23. 25(13):1903-13. 10.1038/sj.onc.1209218 PubMed 16301999