Addgene - MSCV IRES Luciferase Plasmid Data

Plasmid 18760: MSCV IRES Luciferase

Gene/insert name:
None
Vector backbone:
MSCV IRES Luciferase
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral, Luciferase
Backbone size (bp):
6300
5' sequencing primer:
MSCV List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Warren Pear
Sequence: View sequences (2)
Principal Investigator:
Scott Lowe
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

To create MSCV/IRES/LUC, the MSCV/IRES/GFP plasmid was amplified up and digested with NcoI and SalI to remove the GFP. Next, the pGL3-Promoter vector from Promega was amplified up and digested with NcoI and SalI to remove luciferase and the Poly(A) signal. Once completed, the 1.7kb luciferase was ligated into MSCV/IRES to give the MSCV/IRES/LUC plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18760" in your Materials and Methods section.