MSCV IRES Luciferase
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18760||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneMSCV IRES Luciferase
- Backbone size (bp) 6300
Vector typeMammalian Expression, Retroviral, Luciferase
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer MSCV (Common Sequencing Primers)
To create MSCV/IRES/LUC, the MSCV/IRES/GFP plasmid was amplified up and digested with NcoI and SalI to remove the GFP. Next, the pGL3-Promoter vector from Promega was amplified up and digested with NcoI and SalI to remove luciferase and the Poly(A) signal. Once completed, the 1.7kb luciferase was ligated into MSCV/IRES to give the MSCV/IRES/LUC plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV IRES Luciferase was a gift from Scott Lowe (Addgene plasmid # 18760 ; http://n2t.net/addgene:18760 ; RRID:Addgene_18760)