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MSCV IRES Luciferase
(Plasmid #18760)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18760 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV IRES Luciferase
  • Backbone size (bp) 6300
  • Vector type
    Mammalian Expression, Retroviral, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

Depositor Comments

To create MSCV/IRES/LUC, the MSCV/IRES/GFP plasmid was amplified up and digested with NcoI and SalI to remove the GFP. Next, the pGL3-Promoter vector from Promega was amplified up and digested with NcoI and SalI to remove luciferase and the Poly(A) signal. Once completed, the 1.7kb luciferase was ligated into MSCV/IRES to give the MSCV/IRES/LUC plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV IRES Luciferase was a gift from Scott Lowe (Addgene plasmid # 18760 ; http://n2t.net/addgene:18760 ; RRID:Addgene_18760)