|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14887||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberLow Copy
Gene/Insert namegag / pol
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Used for MSCV viral packaging.
The lab emphasizes that this plasmid can be difficult to grow, so always use low copy protocols when preparing DNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:gag/pol was a gift from Tannishtha Reya (Addgene plasmid # 14887 ; http://n2t.net/addgene:14887 ; RRID:Addgene_14887)
For your References section:A role for Wnt signalling in self-renewal of haematopoietic stem cells. Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz L, Nusse R, Weissman IL. Nature. 2003 May 22. 423(6938):409-14. 10.1038/nature01593 PubMed 12717450